Part:BBa_K1582029

Protein Extraction Tag: Linker+sJanus

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 214
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 223
  

Usage and Biology

This year, we focus on solving the protein purification problem in biological lab. As we talked about in the Protein Extraction Kit page in project discription, we aim to replace the lengthy and expensive chromatography with Janus-based aquous two-phase system.

Above all, we take advantage of synthetic biology, using the core of iGEM- BioBrick and RFC Assembly Method, to build a universal purification tag. This tag includes a linker containing TEV protease site and sJanus, designed by the RFC23 method which is specific for fusion protein. Thus, if other teams want to use this system to express and purify their proteins, they could use the standard assembly to make a fusion protein.